Does an expressed sequence tag (EST) library ofSalsola iberica(tumbleweed) help to understand plant responses to environmental stresses?
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چکیده
منابع مشابه
A hitchhiker's guide to expressed sequence tag (EST) analysis
Expressed sequence tag (EST) sequencing projects are underway for numerous organisms, generating millions of short, single-pass nucleotide sequence reads, accumulating in EST databases. Extensive computational strategies have been developed to organize and analyse both small- and large-scale EST data for gene discovery, transcript and single nucleotide polymorphism analysis as well as functiona...
متن کاملIdentifying exoskeleton proteins in the blue crab from an expressed sequence tag (EST) library.
A blue crab (Callinectes sapidus) expressed sequence tag project was designed for multiple purposes including discovery of genes for cuticular (exoskeletal) proteins, some of which may regulate mineralization. One of the expression libraries sequenced was from the hypodermis (the epithelium depositing the cuticle). RNAs used for cDNA synthesis were pooled from arthrodial and mid-dorsal hypoderm...
متن کاملESTExplorer: an expressed sequence tag (EST) assembly and annotation platform
The analysis of expressed sequence tag (EST) datasets offers a rapid and cost-effective approach to elucidate the transcriptome of an organism, but requiring several computational methods for assembly and annotation. ESTExplorer is a comprehensive workflow system for EST data management and analysis. The pipeline uses a 'distributed control approach' in which the most appropriate bioinformatics...
متن کاملPlant responses to environmental stresses—from gene to biotechnology
Increasing global population, urbanization and industrialization are increasing the rate of conversion of arable land into wasteland. Supplying food to an ever-increasing population is one of the biggest challenges that agriculturalists and plant scientists are currently confronting. Environmental stresses make this situation even graver. Despite the induction of several tolerance mechanisms, s...
متن کاملSearching the Expressed Sequence Tag (EST) Databases: Panning for Genes
The genomes of living organisms contain many elements, including genes coding for proteins. The portions of the genes expressed as mature mRNA, collectively known as the transcriptome, represent only a small part of the genome. The expressed sequence tag (EST) databases contain an increasingly large part of the transcriptome of many species. For this reason, these databases are probably the mos...
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ژورنال
عنوان ژورنال: Plant Signaling & Behavior
سال: 2010
ISSN: 1559-2324
DOI: 10.4161/psb.5.11.12837